Microscope Nikon
The Nikon Eclipse Ni-U, beside classical features, is fitted with epifluorescence and four excitation filter blocks (DAPI/Cy3/FITC/Texas Red). It is equipped with 10x/40x/60x/Plan Fluor 100x oil objectives. Images are provided by two cameras:
- A high definition B/W Nikon DS-Qi2
- A medium quality color camera DS-U2
The system is piloted on Win10 by the NIS Element Software – Nikon.
Reservation is mandatory. Costs are 2 CHF per hour.
Maxwell® 16
The Maxwell® 16 extraction and purification robot is the first generation of these device family developed by Promega. This robot is intended for general laboratory use in combination to dedicated kits to perform automatic isolation and purification of DNA, RNA and proteins. 16 samples can be processed in 30 to 45 minutes. Handling and processing is intuitive and rapid.
This robot must be booked in advance on the dedicated reservation site (https://reservations.epfl.ch). The costs is CHF 2.- per run.
Precellys 24 tissue homogenizer
Precellys 24 is the original tissue homogenizer from the Precellys range. Its simplicity and efficiency make the Precellys 24 the best homogenizer solution for routine and R&D projects. The Precellys 24 can homogenize many different samples in 30 seconds. A large range of samples can be processed according to already existing protocols.
Retsch MM400
The MM 400 vibro-mill is a compact, multifunctional bench-top mill specially developed for dry, wet and cryogenic grinding of small amounts of samples.
This model mixes and homogenizes powders in seconds. In addition, it is ideal for the disintegration of biological cells as well as for DNA / RNA extraction and protein extraction. Thanks to its high performance and great flexibility, the MM 400 shredder is a unique product on the market. It is specially equipped for the homogenization of samples in 50 mL plastic tubes, in agreement with the classical DNA extraction kits developed by Qiagen.
Robot MYRA version1.6.15
- The Myra robot is able to prepare a qPCR of 48 tubes in 9 minutes.
- Prepare the qPCR mix
- Serial dilutions for the standard line
- Standardization of sample concentration and pooling of samples (NGS)
- Quick and intuitive programming.
- Do not hesitate to ask us for free training.
One robot run costs 12 CHF and includes 96-well plates for your samples, 1.5ml screw tubes for the qPCR mix, as well as tips are included in the price of the run.
https://www.labgene.ch/pcr-and-qpcr-setup/529-myra-liquid-handling-system.html
Qiacube
Plug-and-play instrument that simplifies and streamlines purification procedures by fully automating all kind of QIAGEN spin-column kits. Automates over 80 QIAGEN kits with over 140 standard protocols for the most frequently used applications. No change of chemistry is required, assuring fast startup and immediate results. The device is equipped for UV light decontamination.
12 samples can be processes at a time. Users bring their own kit and accessories and plasticware are provided.
Example of DNA/RNA extraction kits commonly used with the Qiacube:
- DNeasy PowerSoil kit
- DNeasy PowerSoil Pro kit
- DNeasy PowerWater Sample
- QIAamp® DNA Mini Kit
- DNeasy PowerBiofilm Kit
- Rneasy Mini Kik
This robot must be booked in advance on the dedicated site (https://reservations.epfl.ch).
An introduction to the device is required before any use. Subsequently, support is provided for the choice of the adequate extraction kit and running of the device.
CellDrop FL Unlimited
Dynamic Range: 7 x 102 to 4 x 107 cells/ml
Cell Size Range: 4 to 400 μm
Sample Volume:
3.3 μl @ 30 μm chamber height
10 μl @ 100 μm chamber height (standard)
40 μl @ 400 μm chamber height
Measurement Speed:
Brightfield – 3 seconds @ 1 x 106 cell/ml
Dual Fluorescence – 8.5 seconds @ 1 x 106 cell/ml
Optical Modes: CellDrop FL Dual Fluorescence, Single
Fluorescence, Brightfield, Fluorescence + Brightfield
CellDrop BF: Brightfield only
Sample Surfaces: Optical Sapphire
Brightfield Illumination: LED 530 nm
Fluorescence Illumination: LED 470 nm
Emission Filters:
530 nm +/- 15 nm
620nm +/- 15 nm
The genetic fragment analyzer (Agilent) is a capillary electrophoresis. This single platform runs both sequencing and fragment analysis applications. The analysis of DNA fragments allows several applications, DNA or RNA quantification, genotyping and bacterial identification.
- To prepare the Amplicon Library Sequencing on an Illumina MiSeq
- Very precise
- Analyzes 288 samples in a row without intervention on the machine.
- DNA quality analysis, fragment size, quantity
- 1h30 for 12 samples
- Link to the different existing kits
- The Qubit Flurometer uses fluorescent dyes to determine the concentration of DNA or protein. A standard straight line is used for the assay. It is an accurate and sensitive method.
- Uses 1-20μl of sample per analysis
- Qubit Kit dsDNA HS Assay # Q32851, quantization limit 0.2-100 ng
- Link to the different existing kits
Nano-drop spectrophotometer ND-1000 ThermoScientific
- Fast, economical
- Spectrophotometric assay
- Uses 1-2μl of sample per analysis
- Quantification limit about 5ng / μl
MicPCR Bio molecular system V2.6.5
Quantitative PCR can measure the initial quantity of DNA and the number of amplicons.
Thermocycler PeqLab
To do gradient PCRs, to test different hybridization temperature. Useful when you have problems determining the temperature of your primers.
