The CEL provides services in the field of environmental molecular biology such as sequencing, gene amplification and quantification.
Service
- DNA/RNA extraction and purification
- DNA/RNA quantification
- NGS Library preparation
- 16S, 18S and Microbiome analysis
- Analysis of antibiotic multi-resistant bacteria and resistance genes
- method development on demand
- Technical and scientific support for platform users
- Supports in data analysis and field works
Equipment
Precellys Evolution homogenize or lyse any type of soft or hard sample from animal, human, plant or microorganism in about 30 seconds. The Precellys Evolution allows you to prepare samples in full safety and without cross contamination, for both routine applications and R&D projects. The Precellys Evolution with the cooling unit Cryolys protect thermo-sensitive molecules during the lysis process.
The PULVERISETTE 7 classic line with 2 workstations is ideal for fast, uniform and extremely fine grinding of very small samples down to colloidal fineness of hard, medium hard, brittle and moist samples. Depending on the desired final fineness can the grinding be performed dry or in suspension. it is ideal for the disintegration of biological cells as well as for DNA / RNA extraction and protein extraction.
- The Myra robot is able to prepare a qPCR of 48 tubes in 9 minutes.
- Prepare the qPCR mix
- Serial dilutions for the standard line
- Standardization of sample concentration and pooling of samples (NGS)
- Quick and intuitive programming.
https://biomolecularsystems.com/myra/
The TapeStation 4150 with ScreenTape technology provides fast, easy and reliable nucleic acid analysis for up to 16 samples per analysis. Results are obtained quickly, in 1 to 2 minutes per sample, or less than 20 minutes for 16 samples. It is ideal for quality control (QC) of RNA and DNA samples in Next Generation Sequencing (NGS), microarray and qPCR workflows.
Link to the different existing kits
The Qubit Flex Fluorometer provides accurate and sensitive quantification of DNA, RNA and proteins. It offers the possibility to measure 8 samples in parallel.
The fluorometer features a large display and an intuitive user interface, making it easy to select and run the desired assay, with results displayed in just seconds.
Link to the different existing kits
NanoDrop OneC Spectrophotometer can measure the concentration of DNA, RNA, and protein in a 1-2-µL.
https://www.thermofisher.com/order/catalog/product/ND-ONE-W?SID=srch-srp-ND-ONE-W
Compact Mic qPCR 2 channel can measure the initial quantity of DNA and the number of amplicons.
https://biomolecularsystems.com/mic-qpcr/
Biometra TAdvanced 96 G
To do gradient PCRs, to test different hybridization temperature. Useful when you have problems determining the temperature of your primers.
- heating rate: 6.0°C/sec
- Bloc format: For 96 tubes (0.2 ml), 8-strips, 12-strips or 96-well microplates
- Gradient temperature range: 20°C to 99°C
Biometra TRIO 48
Three Thermocyclers in one for maximum flexibility and high sample throughput
- Bloc formats: 3 x 48 wells for 0.2 ml PCR tubes, 8-strips, 48-well plates
- Temperature range: 3 – 99 °C
- Maximum heating: 6.0°C / sec
