Organic analysis and Vibrational spectroscopy ‒ GR-CEL ‐ EPFL

Agilent LDIR 8700

Thermoelectrocally cooled mercury cadmium telluride (MCT) detector
Infrared quantum cascade laser (QCL) light source
Fast scanning objective
High resolution camera
Spectral range 900 – 1800 cm^-1
Spatial resolution ~1 micron
Spectral resolution ~0.06 cm^-1
reflection modes
Motorised xy-stage controlled by software
Ge Attenuated total reflectance (ATR) objectives equipped with internal pressure sensor and highly accurate and stable column guidance mechanism of precise crystal positioning

Perkin Elmer Spectrum Two

LC: Vanquish (Thermo Fisher Scientific)
High resolution mass spectrometer: Orbitrap Exploris 120 (Thermo Fisher Scientific)
Mass resolution: High mass resolution up to 120,000 (FWHM) at m/z 200 over the mass range m/z 40–3,000
Determination of the elemental compositions based on the accurate m/z values
Scan speed: 22 Hz
Ionisation source: ESI and APCI
Polarity switching: Fast polarity switching with one full experimental cycle acquired at >1.4 Hz
Scan modes: Full scan, MS2 (DDA with Top4 @ 14 Hz), tMS2, tSIM, AIF, DIA and MSX
Full Scan MS – most predominantly used due to comprehensive, high-quality quantitative data provided. Once a mass range is defined to capture all relevant ions, rest assured detection of all compounds in that range will be measured.
Targeted Selected Ion Monitoring (t-SIM) – used for analyses requiring higher sensitivity because you can set a narrow mass range (as narrow as 0.4 Da).
Targeted -MS² (t-MS² formerly Parallel Reaction Monitoring (PRM)) – used for analyses in complex samples requiring additional selectivity. A fragmentation-based acquisition mode, Targeted-MS² provides more selectivity because a high-resolution product ion spectrum is created for only desired precursor ions based on the inclusion list.
Softwares: Compound Discover, m/z Cloud mass spectral library, TraceFinder and Chromeleon