Pre-print and latest publications

Sergey Nazarov, Anass Chiki, Driss Boudeffa and Hilal A. Lashuel. The structural basis of huntingtin (Htt) fibril polymorphism, revealed by cryo-EM of exon 1 Htt fibrils. https://doi.org/10.1101/2021.09.23.461534

Warning

Please note that the publication lists from Infoscience integrated into the EPFL website, lab or people pages are frozen following the launch of the new version of platform. The owners of these pages are invited to recreate their publication list from Infoscience. For any assistance, please consult the Infoscience help or contact support.

2024

Differential Effects of Post-translational Modifications on the Membrane Interaction of Huntingtin Protein

Z. Zhang; C. Gehin; L. A. Abriata; M. Dal Peraro; H. Lashuel

Acs Chemical Neuroscience. 2024-05-16. DOI : 10.1021/acschemneuro.4c00091.

2024

Differential Effects of Post-translational Modifications on the Membrane Interaction of Huntingtin Protein

Post-translational glycosylation diminishes α-synuclein pathology formation

O-GlcNAc forces an α-synuclein amyloid strain with notably diminished seeding and pathology

Author Correction: Fully co-factor-free ClearTau platform produces seeding-competent Tau fibrils for reconstructing pathological Tau aggregates

Effective Inhibition of TDP-43 Aggregation by Native State Stabilization

2023

Recombinant Full-Length TDP-43 Oligomers Retain Their Ability to Bind RNAs, Are Not Toxic, and Do Not Seed TDP-43 Aggregation in Vitro

Deep Learning-Assisted Single-Molecule Detection of Protein Post-translational Modifications with a Biological Nanopore

Development and validation of an expanded antibody toolset that captures alpha-synuclein pathological diversity in Lewy body diseases

Fully co-factor-free ClearTau platform produces seeding-competent Tau fibrils for reconstructing pathological Tau aggregates

Overexpression of human alpha-Synuclein leads to dysregulated microbiome/metabolites with ageing in a rat model of Parkinson disease

Artificial intelligence-coupled plasmonic infrared sensor for detection of structural protein biomarkers in neurodegenerative diseases

Seeding the aggregation of TDP-43 requires post-fibrillization proteolytic cleavage

Neuronal growth on high-aspect-ratio diamond nanopillar arrays for biosensing applications

2022

Prominent astrocytic alpha-synuclein pathology with unique post-translational modification signatures unveiled across Lewy body disorders

Revisiting the specificity and ability of phospho-S129 antibodies to capture alpha-synuclein biochemical and pathological diversity

N-terminal mutant huntingtin deposition correlates with CAG repeat length and symptom onset, but not neuronal loss in Huntington’s disease

Pathological substrate of memory impairment in multiple system atrophy

Opportunities and challenges of alpha-synuclein as a potential biomarker for Parkinson’s disease and other synucleinopathies

On-Demand Nanoliter Sampling Probe for the Collection of Brain Fluid

Structural Basis of Huntingtin Fibril Polymorphism Revealed by Cryogenic Electron Microscopy of Exon 1 HTT Fibrils

Remembering John Q Trojanowski, in his own words: A life dedicated to discovering building blocks and using them to build bridges of knowledge, collaboration, and discovery

A NAC domain mutation (E83Q) unlocks the pathogenicity of human alpha-synuclein and recapitulates its pathological diversity

Pathological Relevance of Post-Translationally Modified Alpha-Synuclein (pSer87, pSer129, nTyr39) in Idiopathic Parkinson’s Disease and Multiple System Atrophy

Comparative Analysis of Total Alpha-Synuclein (alpha SYN) Immunoassays Reveals That They Do Not Capture the Diversity of Modified alpha SYN Proteoforms

Alpha-synuclein as a Potential Biomarker for Parkinson’s Disease and Other Synucleinopathies: Gaps, Challenges, and Opportunities

Non-monotonic fibril surface occlusion by GFP tags from coarse-grained molecular simulations

Revisiting the grammar of Tau aggregation and pathology formation: how new insights from brain pathology are shaping how we study and target Tauopathies

2021

To target Tau pathologies, we must embrace and reconstruct their complexities

Nuclear and cytoplasmic huntingtin inclusions exhibit distinct biochemical composition, interactome and ultrastructural properties

Fatal attraction-The role of hypoxia when alpha-synuclein gets intimate with mitochondria

The Nt17 Domain and its Helical Conformation Regulate the Aggregation, Cellular Properties and Neurotoxicity of Mutant Huntingtin Exon 1

Pharmacological characterization of mutant huntingtin aggregate-directed PET imaging tracer candidates

Alpha-synuclein research: defining strategic moves in the battle against Parkinson’s disease

Investigating Crosstalk Among PTMs Provides Novel Insight Into the Structural Basis Underlying the Differential Effects of Nt17 PTMs on Mutant Httex1 Aggregation

A New Chemoenzymatic Semisynthetic Approach Provides Insight into the Role of Phosphorylation beyond Exon1 of Huntingtin and Reveals N-Terminal Fragment Length-Dependent Distinct Mechanisms of Aggregation

Rethinking protein aggregation and drug discovery in neurodegenerative diseases: Why we need to embrace complexity?

Lewy body-associated proteins: Victims, instigators, or innocent bystanders? The case of AIMP2 and alpha-synuclein

Parkinson mice show functional and molecular changes in the gut long before motoric disease onset

Enforced dimerization between XBP1s and ATF6f enhances the protective effects of the UPR in models of neurodegeneration

Correlative light and electron microscopy suggests that mutant huntingtin dysregulates the endolysosomal pathway in presymptomatic Huntington’s disease

Hypoxia Conditioning as a Promising Therapeutic Target in Parkinson’s Disease?

Reverse engineering Lewy bodies: how far have we come and how far can we go?

Monitoring alpha-synuclein oligomerization and aggregation using bimolecular fluorescence complementation assays: What you see is not always what you get

2020

Site-Specific Phosphorylation of Huntingtin Exon 1 Recombinant Proteins Enabled by the Discovery of Novel Kinases

How specific are the conformation-specific α-synuclein antibodies? Characterization and validation of 16 α-synuclein conformation-specific antibodies using well-characterized preparations of α-synuclein monomers, fibrils and oligomers with distinct structures and morphology

TBK1 phosphorylates mutant Huntingtin and suppresses its aggregation and toxicity in Huntington’s disease models

Half a century of amyloids: past, present and future

Do Lewy bodies contain alpha-synuclein fibrils? and Does it matter? A brief history and critical analysis of recent reports

Phosphorylation of the overlooked tyrosine 310 regulates the structure, aggregation, and microtubule- and lipid-binding properties of Tau

Extent of N-terminus exposure of monomeric alpha-synuclein determines its aggregation propensity

Education and research are essential for lasting peace in Yemen

Unraveling the complexity of amyloid polymorphism using gold nanoparticles and cryo-EM

The busy lives of academics have hidden costs — and universities must take better care of their faculty members

A simple, versatile and robust centrifugation-based filtration protocol for the isolation and quantification of alpha-synuclein monomers, oligomers and fibrils: Towards improving experimental reproducibility in alpha-synuclein research

The process of Lewy body formation, rather than simply α-synuclein fibrillization, is one of the major drivers of neurodegeneration

Site-Specific Hyperphosphorylation Inhibits, Rather than Promotes, Tau Fibrillization, Seeding Capacity, and Its Microtubule Binding

Ultrasensitive quantitative measurement of huntingtin phosphorylation at residue S13

What about faculty?

Pronounced α-synuclein pathology in a seeding-based mouse model is not sufficient to induce mitochondrial respiration deficits in the striatum and amygdala

2019

The Role of Post-translational Modifications on the Energy Landscape of Huntingtin N-Terminus

Phospho-S129 Alpha-Synuclein Is Present in Human Plasma but Not in Cerebrospinal Fluid as Determined by an Ultrasensitive Immunoassay

Single Posttranslational Modifications in the Central Repeat Domains of Tau4 Impact its Aggregation and Tubulin Binding

alpha-Synuclein O-GlcNAcylation alters aggregation and toxicity, revealing certain residues as potential inhibitors of Parkinson’s disease

Chronic corticosterone aggravates behavioral and neuronal symptomatology in a mouse model of alpha-synuclein pathology

Antibody-based methods for the measurement of α-synuclein concentration in human cerebrospinal fluid – method comparison and round robin study

2018

Generation of Native, Untagged Huntingtin Exon1 Monomer and Fibrils Using a SUMO Fusion Strategy

Exploring the role of post-translational modifications in regulating α-synuclein interactions by studying the effects of phosphorylation on nanobody binding

N-terminal Huntingtin (Htt) phosphorylation is a molecular switch regulating Htt aggregation, helical conformation, internalization, and nuclear targeting

Identification and nanomechanical characterization of the fundamental single-strand protofilaments of amyloid α-synuclein fibrils

Resolving molecule-specific information in dynamic lipid membrane processes with multi-resonant infrared metasurfaces

Real-time in-situ secondary structure analysis of protein monolayer with mid-infrared plasmonic nanoantennas

Protein Semisynthesis Provides Access to Tau Disease-Associated Post-translational Modifications (PTMs) and Paves the Way to Deciphering the Tau PTM Code in Health and Diseased States

Combined Multi-Plane Tomographic Phase Retrieval and Stochastic Optical Fluctuation Imaging for 4D Cell Microscopy