Decoding Chromatin Ubiquitylation: A Chemical Biology Perspective

P. Franz; B. Fierz 

Revealing chromatin-specific functions of histone deacylases

C. Moreno-Yruela; B. Fierz 

CENP-A and CENP-B collaborate to create an open centromeric chromatin state

H. Nagpal; A. Ali-Ahmad; Y. Hirano; W. Cai; M. Halic et al. 

Cooperation between bHLH transcription factors and histones for DNA access

A. K. Michael; L. Stoos; P. Crosby; N. Eggers; X. Y. Nie et al. 

Tubulin engineering by semi-synthesis reveals that polyglutamylation directs detyrosination

E. Ebberink; S. Fernandes; G. Hatzopoulos; N. Agashe; P-H. Chang et al. 

De novo design of protein interactions with learned surface fingerprints

P. Gainza Cirauqui; S. Wehrle; A. K. Van Hall-Beauvais; A. Marchand; A. Scheck et al. 

Synthesis of Fluorescent Cyclic Peptides via Gold(I)-Catalyzed Macrocyclization

X-Y. Liu; W. Cai; N. Ronceray; A. Radenovic; B. Fierz et al. 

Multivalent Peptide Ligands To Probe the Chromocenter Microenvironment in Living Cells

N. Guidotti; Á. Eördögh; M. Mivelaz; P. Rivera-Fuentes; B. Fierz 

Release of linker histone from the nucleosome driven by polyelectrolyte competition with a disordered protein

P. O. Heidarsson; D. Mercadante; A. Sottini; D. Nettels; M. B. Borgia et al. 

Multiplexed Single-Molecule Experiments Reveal Nucleosome Invasion Dynamics of the Cas9 Genome Editor

K. Makasheva; L. C. Bryan; C. Anders; S. Panikulam; M. Jinek et al. 

O-GlcNAcylation of High Mobility Group Box 1 (HMGB1) Alters Its DNA Binding and DNA Damage Processing Activities

A. T. Balana; A. Mukherjee; H. Nagpal; S. P. Moon; B. Fierz et al. 

SUV39 SET domains mediate crosstalk of heterochromatic histone marks

A. Stirpe; N. Guidotti; S. J. Northall; S. Kilic; A. Hainard et al. 

Applying Peptide and Protein Synthesis to Study Post-translational Modifications in Epigenetics and Beyond

B. Fierz 

Torsin ATPases influence chromatin interaction of the Torsin regulator LAP1

N. Luithle; J. u. de Bos; R. Hovius; D. Maslennikova; R. T. M. Lewis et al. 

Observing protein interaction dynamics to chemically defined chromatin fibers by colocalization single-molecule fluorescence microscopy

M. Mivelaz; B. Fierz 

The Elusive Structure of Centro-Chromatin: Molecular Order or Dynamic Heterogenetity?

H. Nagpal; B. Fierz 

BAF restricts cGAS on nuclear DNA to prevent innate immune activation

B. Guey; M. Wischnewski; A. Decout; K. Makasheva; M. Kaynak et al. 

Nucleosome Binding by the Lysine Specific Demethylase 1 (LSD1) Enzyme Enables Histone H3 Demethylation

A. Dhall; P. M. M. Shelton; A. Delachat; C. J. A. Leonen; B. Fierz et al. 

Chromatin Fiber Invasion and Nucleosome Displacement by the Rap1 Transcription Factor

M. Mivelaz; A-M. Cao; S. Kubik; S. Zencir; R. Hovius et al. 

Structural mechanism of cGAS inhibition by the nucleosome

G. R. Pathare; A. Decout; S. Glück; S. Cavadini; K. Makasheva et al. 

“Doubly Orthogonal” Labeling of Peptides and Proteins

R. Tessier; J. Ceballos; N. Guidotti; R. Simonet-Davin; B. Fierz et al. 

Chemical and biophysical methods to explore dynamic mechanisms of chromatin silencing

I. Boichenko; B. Fierz 

KAP1 is an antiparallel dimer with a functional asymmetry

G. Fonti; M. J. Marcaida; L. C. Bryan; S. Traeger; A. S. Kalantzi et al. 

A Modular Ligation Strategy for Asymmetric Bivalent Nucleosomes Trimethylated at K36 and K27

N. Guidotti; C. C. Lechner; A. L. Bachmann; B. Fierz 

Revealing chromatin organization in metaphase chromosomes

B. Fierz 

Biophysics of Chromatin Dynamics

B. Fierz; M. G. Poirier 

Single-molecule dynamics and genome-wide transcriptomics reveal that NF-kB (p65)-DNA binding times can be decoupled from transcriptional activation

A. Callegari; C. Sieben; A. Benke; D. M. Suter; B. Fierz et al. 

A bi-terminal protein ligation strategy to probe chromatin structure during DNA damage

S. Kilic; I. Boichenko; C. C. Lechner; B. Fierz 

Engineered Multivalent Sensors to Detect Coexisting Histone Modifications in Living Stem Cells

A. M-F. Delachat; N. Guidotti; A. L. Bachmann; A. C. Meireles-Filho; H. Pick et al. 

Single-molecule FRET reveals multiscale chromatin dynamics modulated by HP1α

S. Kilic; S. Felekyan; O. Doroshenko; I. Boichenko; M. Dimura et al. 

DNA binding by PHF1 prolongs PRC2 residence time on chromatin and thereby promotes H3K27 methylation

J. Choi; A. L. Bachmann; K. Tauscher; C. Benda; B. Fierz et al. 

Dynamic chromatin technologies: from individual molecules to epigenomic regulation in cells

O. Cuvier; B. Fierz 

Single-molecule kinetic analysis of HP1-chromatin binding reveals a dynamic network of histone modification and DNA interactions

L. C. Bryan; D. R. Weilandt; A. L. Bachmann; S. Kilic; C. C. Lechner et al. 

2016 International Symposium on Chemical Biology of the NCCR Chemical Biology Campus Biotech, Geneva 13-15.1.2016

O. Hantschel; B. Fierz 

A two-state activation mechanism controls the histone methyltransferase Suv39h1

M. M. Mueller; B. Fierz; L. Bittova; G. Liszczak; T. W. Muir 

Dynamic Chromatin Regulation from a Single Molecule Perspective

B. Fierz 

Traceless Synthesis of Asymmetrically Modified Bivalent Nucleosomes

C. C. Lechner; N. D. Agashe; B. Fierz 

Multivalency governs HP1α association dynamics with the silent chromatin state

S. Kilic; A. L. Bachmann; L. C. Bryan; B. Fierz 

Molecular and Chemical Mechanism in Epigenetics – Swiss Summer School 2015 July 12–17, 2015, Hotel Kurhaus, Arolla, Switzerland

L. C. Bryan; A. L. Bachmann; B. Fierz 

Engineering chromatin states: Chemical and synthetic biology approaches to investigate histone modification function

H. Pick; S. Kilic; B. Fierz 

Synthetic Chromatin Approaches To Probe the Writing and Erasing of Histone Modifications

B. Fierz 

Accelerated chromatin biochemistry using DNA-barcoded nucleosome libraries

U. T. T. Nguyen; L. Bittova; M. M. Müller; B. Fierz; Y. David et al. 

Testing the diffusing boundary model for the helix-coil transition in peptides

S. Neumaier; A. Reiner; M. Büttner; B. Fierz; T. Kiefhaber 

Stability of Nucleosomes Containing Homogenously Ubiquitylated H2A and H2B Prepared Using Semisynthesis

B. Fierz; S. Kilic; A. R. Hieb; K. Luger; T. W. Muir 

Histone monoubiquitylation position determines specificity and direction of enzymatic cross-talk with histone methyltransferases Dot1L and PRC2

S. J. Whitcomb; B. Fierz; R. K. McGinty; M. Holt; T. Ito et al. 

Chromatin as an expansive canvas for chemical biology

B. Fierz; T. W. Muir 

Histone H2B ubiquitylation disrupts local and higher-order chromatin compaction

B. Fierz; C. Chatterjee; R. K. McGinty; M. Bar-Dagan; D. P. Raleigh et al. 

Disulfide-directed histone ubiquitylation reveals plasticity in hDot1L activation

C. Chatterjee; R. K. McGinty; B. Fierz; T. W. Muir 

Local conformational dynamics in alpha-helices measured by fast triplet transfer

B. Fierz; A. Reiner; T. Kiefhaber 

Loop formation in unfolded polypeptide chains on the picoseconds to microseconds time scale

B. Fierz; H. Satzger; C. Root; P. Gilch; W. Zinth et al. 

End-to-end vs interior loop formation kinetics in unfolded polypeptide chains

B. Fierz; T. Kiefhaber 

Using triplet-triplet energy transfer to measure conformational dynamics in polypeptide chains

B. Fierz; K. Joder; F. Krieger; T. Kiefhaber 

Dynamics of Unfolded Polypeptide Chains

B. Fierz; T. Kiefhaber 

Mechanism of Chromatin Ubiquitination by PRC1 on the Single-Molecule Level

Development of tools and methods for protein identification: From single molecules to in vivo applications

A chemical biology approach to decipher chromatin ubiquitylation by RNF168

Development of multiplexed single-molecule imaging techniques to study chromatin invasion dynamics of chromatin effector proteins

Dynamic Chromatin Invasion and Remodeling by the Transcription Factor Rap1

Development of chemical biology tools to reveal the function and organization of the silenced chromatin state

Modeling biochemical reaction networks in complex intracellular environments

Protein Engineering Tools to Explore the Function of Protein Post-Translational Modifications for Chromatin and Microtubule Cytoskeletal Biology

Dynamic protein-chromatin interactions in heterochromatin and during the DNA damage response on the single molecule level

Deciphering the chromatin binding mechanisms of human HP1 variants at the single-molecule level

Heterochromatin protein 1 recruitment and chromatin conformational dynamics at the single-molecule level

Genetically encoded multivalent sensors to detect bivalent epigenetic modifications in living stem cells