New techniques for high-throughput glycan analysis

This aim of this project is to develop an high-throughput analytical method to identify glycans from biological samples. The challenge of doing so arises from the isomeric complexity of glycans, which is difficult to resolve by any single analytical tool. Our approach combines ultrahigh resolution ion mobility based on structures for lossless ion manipulations (SLIM) with cryogenic ion spectroscopy and mass spectrometry to perform glycan analysis at high throughput.  

See some of our recent papers for details:

Combining Liquid Chromatography and Cryogenic IR Spectroscopy in Real Time for the Analysis of Oligosaccharides

A. H. Abikhodr; S. Warnke; A. Ben Faleh; T. R. Rizzo 

Analytical Chemistry. 2024-01-11. Vol. 96, num. 4, p. 1462-1467. DOI : 10.1021/acs.analchem.3c03578.

Device, system, and method for ion fragmentation by use of an ion mobility device and messenger tagging

S. Warnke; T. Rizzo; A. Ben Faleh 

US2023317442.

2023.

Using Hadamard Transform Multiplexed IR Spectroscopy Together with a Segmented Ion Trap for the Identification of Mobility-Selected Isomers

V. Yatsyna; A. H. Abikhodr; A. Ben Faleh; S. Warnke; T. R. Rizzo 

Analytical Chemistry. 2023-06-12. DOI : 10.1021/acs.analchem.3c01340.

New Approach for the Identification of Isobaric and Isomeric Metabolites

A. Ben Faleh; S. Warnke; T. Van Wieringen; A. H. Abikhodr; T. R. Rizzo 

Analytical Chemistry. 2023-04-29. Vol. 95, num. 18, p. 7118-7126. DOI : 10.1021/acs.analchem.2c04962.

Identification of human milk oligosaccharide positional isomers by combining IMS-CID-IMS and cryogenic IR spectroscopy

A. H. Abikhodr; A. Ben Faleh; S. Warnke; V. Yatsyna; T. R. Rizzo 

Analyst. 2023-04-21. Vol. 148, num. 10, p. 2277-2282. DOI : 10.1039/d3an00407d.

Multistage Ion Mobility Spectrometry Combined with Infrared Spectroscopy for Glycan Analysis

P. Bansal; A. Ben Faleh; S. Warnke; T. R. Rizzo 

Journal Of The American Society For Mass Spectrometry. 2023-03-07. DOI : 10.1021/jasms.2c00361.

A method and system to build an ir fingerprint database for the structural identification of biomolecules

P. Bansal; A. Ben Faleh; R. P. Pellegrinelli; S. Warnke; T. Rizzo 

US2024145027; EP4352735; WO2022259096; WO2022259096.

2022.

A New Approach for Identifying Positional Isomers of Glycans Cleaved from Monoclonal Antibodies

I. Dyukova; A. Ben Faleh; S. Warnke; N. Yalovenko; V. Yatsyna et al. 

Chimia. 2022-04-01. Vol. 76, num. 4, p. 363-363. DOI : 10.2533/chimia.2022.363.

Identification of Mobility-Resolved N-Glycan Isomers

A. Ben Faleh; S. Warnke; P. Bansal; R. P. Pellegrinelli; I. Dyukova et al. 

Analytical Chemistry. 2022-07-07. Vol. 94, num. 28, p. 10101-10108. DOI : 10.1021/acs.analchem.2c01181.

A New Strategy Coupling Ion-Mobility-Selective CID and Cryogenic IR Spectroscopy to Identify Glycan Anomers

R. P. Pellegrinelli; L. Yue; E. Carrascosa; A. Ben Faleh; S. Warnke et al. 

Journal of the American Society for Mass Spectrometry. 2022-04-19. Vol. 33, num. 5, p. 859-864. DOI : 10.1021/jasms.2c00043.

High-Throughput Multiplexed Infrared Spectroscopy of Ion Mobility-Separated Species Using Hadamard Transform

V. Yatsyna; A. H. Abikhodr; A. Ben Faleh; S. Warnke; T. R. Rizzo 

Analytical Chemistry. 2022-02-03. Vol. 94, num. 6, p. 2912–2917. DOI : 10.1021/acs.analchem.1c04843.

Identification of N-glycan positional isomers by combining IMS and vibrational fingerprinting of structurally determinant CID fragments

P. Bansal; A. Ben Faleh; S. Warnke; T. R. Rizzo 

Analyst. 2022-01-21. DOI : 10.1039/d1an01861b.

Identifying Mixtures of Isomeric Human Milk Oligosaccharides by the Decomposition of IR Spectral Fingerprints

A. H. AbiKhodr; V. Yatsyna; A. Ben Faleh; S. Warnke; T. R. Rizzo 

Analytical Chemistry. 2021-10-27. Vol. 93, num. 44, p. 14730-14736. DOI : 10.1021/acs.analchem.1c03190.

Toward High-Throughput Cryogenic IR Fingerprinting of Mobility-Separated Glycan Isomers

S. Warnke; A. Ben Faleh; T. Rizzo 

ACS Measurement Science Au. 2021-09-06. Vol. 1, num. 3, p. 157–164. DOI : 10.1021/acsmeasuresciau.1c00018.

A new approach for identifying positional isomers of glycans cleaved from monoclonal antibodies

I. Dyukova; A. Ben Faleh; S. Warnke; N. Yalovenko; V. Yatsyna et al. 

Analyst. 2021-06-30. Vol. 146, p. 4789-4795. DOI : 10.1039/d1an00780g.

Using SLIM-Based IMS-IMS Together with Cryogenic Infrared Spectroscopy for Glycan Analysis

P. Bansal; V. Yatsyna; A. H. AbiKhodr; S. Warnke; A. Ben Faleh et al. 

Analytical Chemistry. 2020-07-07. Vol. 92, num. 13, p. 9079-9085. DOI : 10.1021/acs.analchem.0c01265.

How General Is Anomeric Retention during Collision-Induced Dissociation of Glycans?

R. P. Pellegrinelli; L. Yue; E. Carrascosa; S. Warnke; A. Ben Faleh et al. 

Journal of the American Chemical Society. 2020-03-16. Vol. 142, num. 13, p. 5948-5951. DOI : 10.1021/jacs.0c00264.

High-throughput cryogenic spectroscopy for glycan analysis

T. Rizzo; S. Warnke; A. Ben Faleh; V. Scutelnic 

US10522337; US2019180997.

2019.

Separation and Identification of Glycan Anomers Using Ultrahigh-Resolution Ion Mobility Spectrometry and Cryogenic Ion Spectroscopy

S. Warnke; A. Ben Faleh; V. Scutelnic; T. Rizzo 

Journal of the American Society for Mass Spectrometry. 2019. Vol. 30, num. 11, p. 2204-2211. DOI : 10.1007/s13361-019-02333-0.

Combining ultra-high resolution ion mobility with cryogenic ion vibrational spectroscopy for the analysis of glycans

S. Warnke; A. Ben Faleh; T. Rizzo 

2019-03-31. National Meeting of the American-Chemical-Society (ACS), Orlando, FL, Mar 31-Apr 04, 2019.

Combining ultra-high resolution ion-mobility spectrometry with cryogenic IR spectroscopy for the study of biomolecular ions

S. Warnke; A. Ben Faleh; R. P. Pellegrinelli; N. Yalovenko; T. R. Rizzo 

Faraday Discussions. 2019. Vol. 217, p. 114-125. DOI : 10.1039/C8FD00180D.

Combining Ultrahigh-Resolution Ion-Mobility Spectrometry with Cryogenic Infrared Spectroscopy for the Analysis of Glycan Mixtures

A. Ben Faleh; S. Warnke; T. Rizzo 

Analytical Chemistry. 2019. Vol. 91, p. 4876-4882. DOI : 10.1021/acs.analchem.9b00659.

The Structure of the Protonated Serine Octamer

V. Scutelnic; M. A. S. Perez; M. Marianski; S. Warnke; A. Gregor et al. 

Journal of the American Chemical Society. 2018-04-11. Vol. 120, num. 24, p. 7554-7560. DOI : 10.1021/jacs.8b02118.
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