Life on earth at all scales (societies, behavior, physiology, molecular functions) is temporally organized along the 24h daily cycle. This project builds on our longstanding interest to combine computational and experimental approaches to understand gene regulatory mechanisms underlying circadian rhythms, and notably their impacts on temporal liver physiology. In particular, we aim at integrating time resolved functional genomics datasets in mouse organs to model how the circadian clock and/or environmental cycles impinge on gene regulation across multiple levels, from transcription to translation to protein accumulation. We will focus on models combining multiple types of RNA-seq and other omics measurement to dissect the dynamics of gene expression, including the mechanisms governing RNA transcription, accumulation and its translation. Implications of clock function and feeding rhythms on the processes, including the consequence on physiology and liver function, will be analyzed. The work is dry (although, depending on interest of the student, performing validation experiments would be a plus) and highly interdisciplinary, combining concepts/tools from gene regulation, bioinformatics, applied math and computer science.
Links to representative recent publications form our lab, illustrating the questions and methodologies that will be further developed:
- Mermet, Genes Development 2018, http://genesdev.cshlp.org/content/32/5-6/347
- Yeung, Genome Research 2018, https://genome.cshlp.org/content/28/2/182
- Wang, PNAS 2018, https://www.pnas.org/content/115/8/E1916
- Gobet, bioRxiv 2019, https://www.biorxiv.org/content/10.1101/551838v1
For any information on available current or future positions, please contact [email protected]