Sizes and size distributions of macromolecules and particles

 

  • Gel Permeation Chromatography / Size Exclusion Chromatography (GPC/SEC)
The platform offers three different GPCs. They are located in LP.
 

Name Eluent Detector
Agilent 390-MDS THF RI, Viscometer, Dual-Angle Light Scattering
PSS-Polymer SECcurity² DMAc + 0.1 %w/w LiBr

RI, UV, Viscometer, Multi-Angle Light Scattering


 

If you need GPC measurements, please contact Helin Findik and make the order through PPMS.

 

 

The DLS is a Nano ZS from Malvern.

It is located in MXD 137.

If you want a training, please request it on PPMS.

Specifications:

  • Size measurement from 0.3nm (diameter) to 10 microns using patented NIBS (Non-Invasive Back Scatter) technology
  • Molecular weight measurement down to 980 Da
  • Zeta potential of proteins and particles using patented M3-PALS technology
  • Second virial coefficient measurement for solubility
  • Automated temperature trend measurement
  • Sample concentrations from 0.1ppm to 40%w/v
  •  Protein size measurement as dilute as 0.1mg/mL (Lysozyme)
  • Laser Wavelength: 633nm (red)