Highlight:
The true shape of dendritic spines – December 2020
Using cryo fixation, the BioEM facility shows how dendritic spines are altered by chemical fixation. The dendritic spine is the site where the majority of excitatory connections are found in the adult brain. There size and shape are important determinants of their function. A detailed morphological analysis now published in eLife shows few changes, however, cryo-fixed spines have significantly thinner necks.
TEM in Parkinson’s disease
The group of Hilal Lashuel publishes an extensive study in the journal PNAS in which the progression of Lewy body formation is tracked in cells. This includes transmission electron microscopy with immunocytochemistry. See the EPFL news here. The research article can be viewed here.
Block face scanning EM in Alzheimer’s Disease
A study lead by Gilles Bonvento (Paris) explores astrocytic metabolism and mechanistic link that connects defective glycolysis in astrocytes to impaired l-serine production and reduced neuronal function. Supplementation with L-serine in a mouse model of Alzheimer’s restores cognitive deficits. This study used the block-face scanning electron microscopy services offered by the BioEM Facility. The study is published in the journal Cell Metabolism, and can be viewed here.
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2023
Mechanopathology of biofilm-like Mycobacterium tuberculosis cords
Cell. 2023-11-09. Vol. 186, num. 23, p. 5135-+. DOI : 10.1016/j.cell.2023.09.016.Stable isotope labeling and ultra-high-resolution NanoSIMS imaging reveal alpha-synuclein-induced changes in neuronal metabolism in vivo
Acta Neuropathologica Communications. 2023-09-29. Vol. 11, num. 1, p. 157. DOI : 10.1186/s40478-023-01608-8.Large-scale morphometry of the subarachnoid space of the optic nerve
Fluids And Barriers Of The Cns. 2023-03-21. Vol. 20, num. 1, p. 21. DOI : 10.1186/s12987-023-00423-6.Dynamics of CLIMP-63 S-acylation control ER morphology
Nature Communications. 2023-01-17. Vol. 14, num. 1. DOI : 10.1038/s41467-023-35921-6.Correlative light and volume electron microscopy to study brain development
Microscopy. 2023-01-09. DOI : 10.1093/jmicro/dfad002.2022
Retromer deficiency in Tauopathy models enhances the truncation and toxicity of Tau
Nature Communications. 2022-08-27. Vol. 13, num. 1, p. 5049. DOI : 10.1038/s41467-022-32683-5.ADAMTS18(+) villus tip telocytes maintain a polarized VEGFA signaling domain and fenestrations in nutrient-absorbing intestinal blood vessels
Nature Communications. 2022-07-09. Vol. 13, num. 1, p. 3983. DOI : 10.1038/s41467-022-31571-2.A subpopulation of cortical VIP-expressing interneurons with highly dynamic spines
Communications Biology. 2022-04-13. Vol. 5, num. 1, p. 352. DOI : 10.1038/s42003-022-03278-z.2021
Nuclear and cytoplasmic huntingtin inclusions exhibit distinct biochemical composition, interactome and ultrastructural properties
Nature Communications. 2021-11-12. Vol. 12, num. 1, p. 6579. DOI : 10.1038/s41467-021-26684-z.Early invasion of the bladder wall by solitary bacteria protects UPEC from antibiotics and neutrophil swarms in an organoid model
Cell Reports. 2021-07-20. Vol. 36, num. 3, p. 109351. DOI : 10.1016/j.celrep.2021.109351.Dynamic persistence of UPEC intracellular bacterial communities in a human bladder-chip model of urinary tract infection
eLife. 2021-07-05. Vol. 10, p. e66481. DOI : 10.7554/eLife.66481.Deep Active Surface Models
2021-06-24. Conference on Computer Vision and Pattern Recognition (CVPR), Virtual, June 21-25, 2021. p. 11652-11661. DOI : 10.1109/CVPR46437.2021.01148.Maturation of Complex Synaptic Connections of Layer 5 Cortical Axons in the Posterior Thalamic Nucleus Requires SNAP25
Cerebral Cortex. 2021-05-01. Vol. 31, num. 5, p. 2625-2638. DOI : 10.1093/cercor/bhaa379.3D Ultrastructure of Synaptic Inputs to Distinct GABAergic Neurons in the Mouse Primary Visual Cortex
Cerebral Cortex. 2021-05-01. Vol. 31, num. 5, p. 2610-2624. DOI : 10.1093/cercor/bhaa378.Toward Biophysical Mechanisms of Neocortical Computation after 50 Years of Barrel Cortex Research
Function. 2021-01-01. Vol. 2, num. 1, p. zqaa046. DOI : 10.1093/function/zqaa046.Mitofusin-2 in nucleus accumbens regulates anxiety and depression-like behaviors through mitochondrial and neuronal actions
Biological Psychiatry. 2021. Vol. 89, num. 11, p. 1024-1026. DOI : 10.1016/j.biopsych.2020.12.003.2020
Ultrastructural comparison of dendritic spine morphology preserved with cryo and chemical fixation
Elife. 2020-12-04. Vol. 9, p. e56384. DOI : 10.7554/eLife.56384.Gas cluster ion beam SEM for imaging of large tissue samples with 10 nm isotropic resolution
Nature Methods. 2020-11-18. Vol. 17, num. 1, p. 68-71. DOI : 10.1038/s41592-019-0641-2.Nano-imaging trace elements at organelle levels in substantia nigra overexpressing alpha-synuclein to model Parkinson’s disease
Communications Biology. 2020-07-09. Vol. 3, num. 1, p. 364. DOI : 10.1038/s42003-020-1084-0.Combined deletion of Glut1 and Glut3 impairs lung adenocarcinoma growth
Elife. 2020-06-23. Vol. 9, p. e53618. DOI : 10.7554/eLife.53618.Somatostatin enhances visual processing and perception by suppressing excitatory inputs to parvalbumin-positive interneurons in V1
Science Advances. 2020-04-01. Vol. 6, num. 17, p. eaaz0517. DOI : 10.1126/sciadv.aaz0517.Dysfunction of homeostatic control of dopamine by astrocytes in the developing prefrontal cortex leads to cognitive impairments
Molecular Psychiatry. 2020-04-01. Vol. 25, num. 4, p. 732-749. DOI : 10.1038/s41380-018-0226-y.Impairment of Glycolysis-Derived L-Serine Production in Astrocytes Contributes to Cognitive Deficits in Alzheimer’s Disease
Cell Metabolism. 2020-03-03. Vol. 31, num. 3, p. 503-517.e8. DOI : 10.1016/j.cmet.2020.02.004.The process of Lewy body formation, rather than simply α-synuclein fibrillization, is one of the major drivers of neurodegeneration
Proceedings of the National Academy of Sciences of United States of America. 2020-02-19. p. 201913904. DOI : 10.1073/pnas.1913904117.Voxel2Mesh: 3D Mesh Model Generation from Volumetric Data
2020. International Conference On Medical Image Computing & Computer Assisted Intervention (MICCAI), Lima, Peru, 4-8 OCTOBER 2020.Amygdala GluN2B-NMDAR dysfunction is critical in abnormal aggression of neurodevelopmental origin induced by St8sia2 deficiency
Mol Psychiatry. 2020. Vol. 25, num. 9, p. 2144-61. DOI : 10.1038/s41380-018-0132-3.2019
Probabilistic Atlases to Enforce Topological Constraints
2019-10-10. 22rd International Conference On Medical Image Computing & Computer Assisted Intervention, Shenzhen, China, 2019-10-13. DOI : 10.1007/978-3-030-32239-7_25.Synaptic proximity enables NMDAR signalling to promote brain metastasis
Nature. 2019-09-26. Vol. 573, num. 7775, p. 526-531. DOI : 10.1038/s41586-019-1576-6.The RNA-Binding Protein PUM2 Impairs Mitochondrial Dynamics and Mitophagy During Aging
Molecular Cell. 2019-02-21. Vol. 73, num. 4, p. 775-787.e10. DOI : 10.1016/j.molcel.2018.11.034.Diversity of Cortico-descending Projections: Histological and Diffusion MRI Characterization in the Monkey
Cerebral Cortex. 2019-02-01. Vol. 29, num. 2, p. 788-801. DOI : 10.1093/cercor/bhx363.Cellular Uptake and Intracellular Trafficking of Poly(N-(2-Hydroxypropyl) Methacrylamide)
Biomacromolecules. 2019. Vol. 20, num. 1, p. 231-242. DOI : 10.1021/acs.biomac.8b01372.2018
In vivo modeling of human neuron dynamics and Down syndrome
Science. 2018-11-16. Vol. 362, num. 6416, p. eaau1810. DOI : 10.1126/science.aau1810.Block Face Scanning Electron Microscopy of Fluorescently Labeled Axons Without Using Near Infra-Red Branding
Frontiers In Neuroanatomy. 2018-11-06. Vol. 12, p. 88. DOI : 10.3389/fnana.2018.00088.Ultrastructural basis of strong unitary inhibition in a binaural neuron
Journal Of Physiology-London. 2018-10-15. Vol. 596, num. 20, p. 4969-4982. DOI : 10.1113/JP276015.Locally coordinated synaptic plasticity of visual cortex neurons in vivo
Science. 2018-06-22. Vol. 360, num. 6395, p. 1349-1354. DOI : 10.1126/science.aao0862.NeuroMorph: A Software Toolset for 3D Analysis of Neurite Morphology and Connectivity
Frontiers in Neuroanatomy. 2018. Vol. 12, p. 59. DOI : 10.3389/fnana.2018.00059.The effects of aging on neuropil structure in mouse somatosensory cortex-A 3D electron microscopy analysis of layer 1
PLOS ONE. 2018. Vol. 13, num. 7, p. e0198131. DOI : 10.1371/journal.pone.0198131.Neuron Segmentation With High-Level Biological Priors
IEEE TRANSACTIONS ON MEDICAL IMAGING. 2018. Vol. 37, num. 4, p. 829-839. DOI : 10.1109/TMI.2017.2712360.Molecular insights into Vibrio cholerae’s intra-amoebal host-pathogen interactions
Nature Communications. 2018. Vol. 9, p. 3460. DOI : 10.1038/s41467-018-05976-x.2017
Computer assisted detection of axonal bouton structural plasticity in in vivo time-lapse images
Elife. 2017. Vol. 6, p. e29315. DOI : 10.7554/eLife.29315.The Differential Distribution of RAPTA-T in Non-Invasive and Invasive Breast Cancer Cells Correlates with Its Anti-Invasive and Anti-Metastatic Effects
International Journal Of Molecular Sciences. 2017. Vol. 18, num. 9, p. 1869. DOI : 10.3390/ijms18091869.Homeostatic control of dopamine by astrocytes in the postnatal maturation of the prefrontal cortex
2017. ISN-ESN Meeting, Paris, FRANCE, AUG 20-24, 2017. p. 18-18.Identification of aminopyrimidine-sulfonamides as potent modulators of Wag31-mediated cell elongation in mycobacteria
Molecular Microbiology. 2017. Vol. 103, num. 1, p. 13-25. DOI : 10.1111/mmi.13535.Parkin functionally interacts with PGC-1 proportional to to preserve mitochondria and protect dopaminergic neurons
Human Molecular Genetics. 2017. Vol. 26, num. 3, p. 582-598. DOI : 10.1093/hmg/ddw418.Multicut brings automated neurite segmenation closer to human performance
Nature Methods. 2017. Vol. 14, num. 2, p. 101-102. DOI : 10.1038/nmeth.4151.2016
Cell Division by Longitudinal Scission in the Insect Endosymbiont
mBio. 2016. Vol. 7, num. 4, p. e00881-16. DOI : 10.1128/mBio.00881-16.Multi-Modal Optical Imaging of the Cerebellum in Animals
2016. 7th International Symposium of the Society-for-Research-on-the-Cerebellum (SRC), Brussels, BELGIUM, MAY 08-10, 2015. p. 18-20. DOI : 10.1007/s12311-015-0730-4.2015
C6-O-03Quantifying adult brain ultrastructure using focussed ion beam scanning electron microscopy
Microscopy. 2015. Vol. 64, p. i74-i74. DOI : 10.1093/jmicro/dfv203.NanoSIMS analysis of an isotopically labelled organometallic ruthenium(II) drug to probe its distribution and state in vitro
Chemical Communications. 2015. Vol. 51, num. 92, p. 16577-16577. DOI : 10.1039/c5cc90487k.NanoSIMS analysis of an isotopically labelled organometallic ruthenium(II) drug to probe its distribution and state in vitro
Chemical Communications. 2015. Vol. 51, num. 92, p. 16486-16489. DOI : 10.1039/c5cc06983a.PGC-1 alpha activity in nigral dopamine neurons determines vulnerability to alpha-synuclein
Acta Neuropathologica Communications. 2015. Vol. 3, p. 16. DOI : 10.1186/s40478-015-0200-8.Imaging the time-integrated cerebral metabolic activity with subcellular resolution through nanometer-scale detection of biosynthetic products deriving from (13)C-glucose
Journal of chemical neuroanatomy. 2015. Vol. 69, p. 7-12. DOI : 10.1016/j.jchemneu.2015.09.003.Delayed and Temporally Imprecise Neurotransmission in Reorganizing Cortical Microcircuits
Journal Of Neuroscience. 2015. Vol. 35, num. 24, p. 9024-9037. DOI : 10.1523/Jneurosci.4583-14.2015.Ultrastructural analysis of adult mouse neocortex comparing aldehyde perfusion with cryo fixation
Elife. 2015. Vol. 4, p. e05793. DOI : 10.7554/eLife.05793.A single epidermal stem cell strategy for safe ex vivo gene therapy
EMBO molecular medicine. 2015. Vol. 7, num. 4, p. 380-93. DOI : 10.15252/emmm.201404353.Multiphoton microscopy in brain imaging
2015. Conference on Multiphoton Microscopy in the Biomedical Sciences XV, San Francisco, CA, FEB 08-10, 2015. DOI : 10.1117/12.2084183.Cold shock protects the brain
Nature. 2015. Vol. 518, num. 7538, p. 177-178. DOI : 10.1038/nature14195.Ultrastructurally smooth thick partitioning and volume stitching for large-scale connectomics
Nature Methods. 2015. Vol. 12, num. 4, p. 319-U55. DOI : 10.1038/Nmeth.3292.Modeling Brain Circuitry over a Wide Range of Scales
Frontiers in Neuroanatomy. 2015. Vol. 9, p. 42. DOI : 10.3389/fnana.2015.00042.Learning Structured Models for Segmentation of 2D and 3D Imagery
IEEE Transactions on Medical Imaging. 2015. Vol. 34, num. 5, p. 1096-1110. DOI : 10.1109/TMI.2014.2376274.Imaging liver and brain glycogen metabolism at the nanometer scale
Nanomedicine: Nanotechnology, Biology and Medicine. 2015. Vol. 11, num. 1, p. 239-245. DOI : 10.1016/j.nano.2014.09.007.NeuroMorph: A Toolset for the Morphometric Analysis and Visualization of 3D Models Derived from Electron Microscopy Image Stacks
Neuroinformatics. 2015. Vol. 13, num. 1, p. 83-92. DOI : 10.1007/s12021-014-9242-5.2014
Efficient scanning for em based target localization
WO2014001157; US8588509.
2014.Nanometer-scale molecular imaging of cerebral metabolic substrate incorporation.
Life Sciences Switzerland Annual Meeting, Lausanne, Switzerland, February 4-5, 2014.Correlative In Vivo 2-Photon Beam Scanning Electron Microscopy: 3D Analysis of Neuronal Ultrastructure
Correlative Light And Electron Microscopy II; San Diego: Elsevier Academic Press Inc, 2014. p. 339-361.SAS-1 Is a C2 Domain Protein Critical for Centriole Integrity in C. elegans
Plos Genetics. 2014. Vol. 10, num. 11, p. e1004777. DOI : 10.1371/journal.pgen.1004777.Conditional expression of Parkinson’s disease-related R1441C LRRK2 in midbrain dopaminergic neurons of mice causes nuclear abnormalities without neurodegeneration
Neurobiology Of Disease. 2014. Vol. 71, p. 345-358. DOI : 10.1016/j.nbd.2014.08.027.Semiautomated correlative 3D electron microscopy of in vivo-imaged axons and dendrites
Nature Protocols. 2014. Vol. 9, num. 6, p. 1354-1366. DOI : 10.1038/nprot.2014.101.New tool for studying the biosynthetic role of glucose in brain at the nanometer scale
11th International Conference on Brain Energy Metabolism, Helsinge, Denmark, May 11-14, 2014.Connexin 30 sets synaptic strength by controlling astroglial synapse invasion
Nature Neuroscience. 2014. Vol. 17, num. 4, p. 549-U104. DOI : 10.1038/nn.3662.FOXO3 determines the accumulation of alpha-synuclein and controls the fate of dopaminergic neurons in the substantia nigra
Human Molecular Genetics. 2014. Vol. 23, num. 6, p. 1435-1452. DOI : 10.1093/hmg/ddt530.The Relationship between PSD-95 Clustering and Spine Stability In Vivo
Journal Of Neuroscience. 2014. Vol. 34, num. 6, p. 2075-2086. DOI : 10.1523/Jneurosci.3353-13.2014.Pansynaptic Enlargement at Adult Cortical Connections Strengthened by Experience
Cerebral Cortex. 2014. Vol. 24, num. 2, p. 521-531. DOI : 10.1093/cercor/bhs334.2013
Universal sample holder
US2015022807; WO2013136260.
2013.Is EM dead?
Journal Of Cell Science. 2013. Vol. 126, num. 20, p. 4545-4552. DOI : 10.1242/jcs.124123.BMP signaling specifies the development of a large and fast CNS synapse
Nature Neuroscience. 2013. Vol. 16, num. 7, p. 856-U109. DOI : 10.1038/nn.3414.Correlative in vivo 2 photon and focused ion beam scanning electron microscopy of cortical neurons
PloS One. 2013. Vol. 8, num. 2, p. e57405. DOI : 10.1371/journal.pone.0057405.Increased axonal bouton dynamics in the aging mouse cortex
Proceedings of the National Academy of Sciences of the United States of America. 2013. Vol. 110, num. 16, p. E1514-23. DOI : 10.1073/pnas.1218731110.Imaging green fluorescent protein-labeled neurons using light and electron microscopy
Cold Spring Harbor protocols. 2013. Vol. 2013, num. 6, p. 542-50. DOI : 10.1101/pdb.prot075127.In vivo single branch axotomy induces GAP-43-dependent sprouting and synaptic remodeling in cerebellar cortex
Proceedings of the National Academy of Sciences of the United States of America. 2013. Vol. 110, num. 26, p. 10824-9. DOI : 10.1073/pnas.1219256110.In-vivo single neuron axotomy triggers axon regeneration to restore synaptic density in specific cortical circuits
Nature Communications. 2013. Vol. 4, p. 2038. DOI : 10.1038/ncomms3038.Flash Scanning Electron Microscopy
2013. 16th Conference on Medical Image Computing and Computer Assisted Intervention (MICCAI), Nagoya, Japan, September 22-26, 2013.Mitonuclear protein imbalance as a conserved longevity mechanism
Nature. 2013. Vol. 497, num. 7450, p. 451-457. DOI : 10.1038/nature12188.Altered Synaptic Dynamics during Normal Brain Aging
Journal Of Neuroscience. 2013. Vol. 33, num. 9, p. 4094-4104. DOI : 10.1523/Jneurosci.4825-12.2013.Learning Context Cues for Synapse Segmentation
IEEE Transactions on Medical Imaging. 2013. Vol. 32, num. 10, p. 1864–1877. DOI : 10.1109/Tmi.2013.2267747.2012
3D ultrastructural study of tissues by serial block-face scanning electron microscopy
2012. 39th Annual Meeting of the Society-for-Cutaneous-Ultrastructure-Research (CUR). p. E1-E1.Efficient Scanning for EM Based Target Localization
2012. 15th International Conference on Medical Image Computing and Computer Assisted Intervention, Nice, France, October, 2012. p. 337-344. DOI : 10.1007/978-3-642-33454-2_42.Learning Context Cues for Synapse Segmentation in EM Volumes
2012. International Conference on Medical Image Computing and Computer Assisted Intervention (MICCAI). p. 585-592. DOI : 10.1007/978-3-642-33415-3_72.Analysis of centriole elimination during C. elegans oogenesis
Development. 2012. Vol. 139, num. 9, p. 1670-1679. DOI : 10.1242/dev.075440.Nigrostriatal overabundance of alpha-synuclein leads to decreased vesicle density and deficits in dopamine release that correlate with reduced motor activity
Acta Neuropathologica. 2012. Vol. 123, num. 5, p. 653-669. DOI : 10.1007/s00401-012-0963-y.GABA Signaling Promotes Synapse Elimination and Axon Pruning in Developing Cortical Inhibitory Interneurons
Journal Of Neuroscience. 2012. Vol. 32, p. 331-343. DOI : 10.1523/JNEUROSCI.3189-11.2012.Supervoxel-Based Segmentation of Mitochondria in EM Image Stacks with Learned Shape Features
IEEE Transactions on Medical Imaging. 2012. Vol. 31, num. 2, p. 474-486. DOI : 10.1109/TMI.2011.2171705.2011
Focused Ion Beam Nano-Tomography Using Different Detectors
Microscopy and Microanalysis. 2011. Vol. 17, num. S2, p. 882-883. DOI : 10.1017/S1431927611005289.Automated Detection and Segmentation of Synaptic Contacts in Nearly Isotropic Serial Electron Microscopy Images
Plos One. 2011. Vol. 6, num. 10, p. e24899. DOI : 10.1371/journal.pone.0024899.2010
FIB-SEM Nanotomography in Materials and Life Science at EPFL
Microscopy and Microanalysis. 2010. Vol. 16, num. S2, p. 226-227. DOI : 10.1017/S1431927610059623.Alpha-Synuclein Animal Models
2010. 2nd World Parkinson Congress, Glasgow, UK, September 28 – October 1, 2010. p. S606-S606.Supervoxel-Based Segmentation of EM Image Stacks with Learned Shape Features
2010