Cryo-ultramicrotomy

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Cryo-Ultramicrotomy enables to make thin sections  of constant thickness (~20-200 nm) with very soft materials that cannot be cut at ambient temperature.

A cryo- chamber filled with liquid nitrogen is added to the ultramicrotome. Sample, knife and surrounding area are cooled down to the required temperature.

This method allows any sample orientation.

 

CIME ultramicrotomes:

1. Leica UC 7 + Cryo chamber FC 7
2. Old Reichert Young

 

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