We provide access to fully equipped P1 and P2 cell culture laboratories and support for the development of organoid and iPSC models including:
• Teaching basic organoid and iPSC culture methods to researchers
• Centralising the purchase and distribution of common culture reagents
• Repository of organoids and iPSCs
Resources
- Fully equipped P1 and P2 cell culture rooms
- Dissection and quarantine room
- Media, basal membrane extracts and culture supplements
- Electroporator (Nepagene NEPA21)
- Cryofreezer (Kryo 560M-16M)
- Mouse organoid repository
How to get access?
- Access request
- Discussion of the planned project and its execution
- Acceptance of the rules
- Training
- Access to reservation system
Services
- Portfolio of protocols
- Support during establishment of new protocols
- Cryopreservation
- Mycoplasma testing
Training
- Human and mouse organoid maintenance (feeding and passaging)
- Human and mouse organoid cryopreservation (freezing and thawing)
- Fixation and whole mount IF staining of organoids
- DNA/RNA/protein isolation from organoids
- Electroporation
Useful links
Coming soon
- Organoid gene modification
- Derivation of mouse organoids
- Human/mouse iPSC and organoid repository
- iPSC maintenance and differentiation