Mass Cytometry, a hybrid technology between flow cytometry and mass spectrometry, is a powerful single-cell proteomics technology that allows for the simultaneous detection and measurement of 40+ features for every single cell analyzed. Mostly based on immunophenotyping, this technology is based on the use of antibodies coupled to purified isotopes from rare-earth metals. Because these isotopes have discrete and specific atomic masses and as the machine has a 1-Da resolution, mass cytometry overcomes the limitations of conventional flow cytometry caused by spectral overlap between fluorescent molecules.
Our mass cytometer is a CyTOF2 upgraded to the latest generation, called Helios.
Please check the Fluidigm website for further information about mass spectrometry.
The CyTOF is located in room AI 9126, in the basement of AI building.
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Articles published in collaboration with the EPFL FCCF and using Mass Cytometry:
- Characterization of mesoangioblast cell fate and improved promyogenic potential of a satellite cell-like subpopulation upon transplantation in dystrophic murine muscles. Mavoungou LO, Neuenschwander S, Pham U, Iyer PS, Mermod. N. Stem Cell Res. 2019 Dec;41:101619. doi: 10.1016/j.scr.2019.101619. Epub 2019 Oct 25.
- Drug-induced expression of the RNA-binding protein HuR attenuates the adaptive response to BRAF inhibition in melanoma. Merat R, Bugi-Marteyn A, Wrobel LJ, Py C, Daali Y, Schwärzler C, Liaudet N. Biochem Biophys Res Commun. 2019 Sep 17;517(2):181-187. doi: 10.1016/j.bbrc.2019.06.154. Epub 2019 Jul 3.
- Overexpression of the human antigen R suppresses the immediate paradoxical proliferation of melanoma cell subpopulations in response to suboptimal BRAF inhibition. Fernandez M, Sutterlüty-Fall H, Schwärzler C, Lemeille S, Boehncke WH, Merat R. Cancer Med. 2017 Jul;6(7):1652-1664. doi: 10.1002/cam4.1091. Epub 2017 Jun 1.
Mass Cytometry experiments and analyses are run as a service provided by the facility, in a collaborative way.
Research groups interested in using the mass cytometer must initiate discussion with Sènan D’Almeida and Miguel Garcia and must therefore send requests to the following email address:
A list of videos made by the EPFL FCCF, as well as useful articles, webinars and educational material can be requested by contacting the Mass Cytometry staff via [email protected].