Gene Expression Core Facility
![](https://www.epfl.ch/research/facilities/gene-expression-core-facility/wp-content/uploads/2021/10/main-image-GECF-1536x864.jpeg)
DNA molecules (Adobe education license)
Mission
We provide a diverse set of tools for the functional and quantitative analysis of genomes and transcriptomes, both at single cells level or on bulk cell populations. This comprises in particular next-generation sequencing, classical or high-throughput microfluidics-based qPCR technologies, digital PCR, and spatial transcriptomics analyses. In addition, we enable manipulation of the cellular gene expression program by providing different genome-wide collections of ORFs. Because new technologies constantly emerge, we are eager to discuss means to further improve and develop our toolbox.
Expertise
![](https://www.epfl.ch/research/facilities/gene-expression-core-facility/wp-content/uploads/2021/10/Next-generation-sequencing-384x216.jpeg)
Next generation sequencing
Illumina (MiSeq, NextSeq, NovaSeq) or Elembio (Aviti) short reads. Oxford Nanopore long reads (MinION, Promethion)
![](https://www.epfl.ch/research/facilities/gene-expression-core-facility/wp-content/uploads/2021/10/single-cells-384x216.jpeg)
Single cells genomics/ transcriptomics
10X Genomics ChromiumX, Parse…
![](https://www.epfl.ch/research/facilities/gene-expression-core-facility/wp-content/uploads/2021/10/SPatial-transcriptomics-384x216.png)
Spatial Transcriptomics
10X Genomics Visium / CytAssist
![](https://www.epfl.ch/research/facilities/gene-expression-core-facility/wp-content/uploads/2021/10/quantitative-PCR-384x216.jpeg)
Quantitative and digital PCR
Classical qPCR (Applied Biosystems QuantStudio), microfluidics-based qPCR (Fluidigm BioMark), and digital PCR (Qiagen Qiacuity)
![](https://www.epfl.ch/research/facilities/gene-expression-core-facility/wp-content/uploads/2021/10/capillary-electrophoresis-384x216.jpg)
Nucleic acids QC
Profiling (TapeStation 4200 and Fragment Analyzer) and quantification (Qubit, Nanodrop One)
![](https://www.epfl.ch/research/facilities/gene-expression-core-facility/wp-content/uploads/2021/10/ORFs-collection-384x216.jpeg)
ORF & cDNA Collections
We manage and distribute several large collections of ORFs
News
July 2024
- New price list available here.
April 2024
- Visium HD is here! Spatial transcriptomics resolution is now down to single cell scale. More info here!
March 2024
- Our new Aviti sequencer is operational. Please inquire. More news soon anyway.
January 2024
- A warm welcome to our new collaborator Michael Stock.
November 2023
- New price list available here, with decrease of fixed RNA 10XG (FLEX) prices
October 2023
- Launch of our PromethION long reads service.
July 2023
- New price list available here! Many new services included. Prices decrease of NovaSeq runs.
June 2023
- We have launched the 10X Genomics FLEX (fixed RNA profiling) service, based on fixed cells. Ideal for evening or weekend processing of samples since fixed cells can be stored.
April 2023
- We have just launched a HiC libraries prep service, based on Arima workflow. Inquire for pricing and details.
- A new libraries submission form is available here (together with updated guidelines).
February 2023
- Our new CytAssist instrument now allows us to process FFPE slides for spatial transcriptomics analysis of human and mouse tissues with improved sensitivity, flexibility and robustness.
- Our new QIAcuity One 5plex digital PCR system is up and running.
2022
- From now on please use our team email address [email protected] for projects submissions and other demands.
- New versions of our libraries submission form/guidelines are available here.
- We have launched our NovaSeq 6000 service! Please inquire.
2021
- We have launched a Visium FFPE spatial transcriptomics service, in collaboration with HCF.
- A NovaSeq 6000 will soon replace our HiSeq 4000! More news in December.
- A warm welcome to our new collaborator Chiara Sartori.
- The replacement process for our HiSeq 4000 sequencer has begun, more news in 2 months.
- Our new 10X Genomics single cell multiome (scATAC-seq+scRNA-seq) and CellPlex services are operational.
- Our new QuantStudio 6 instrument is now operational.
- Our new web tool CloneMap for ORFs browsing and QC is operational (thanks Fabrice David).
- A warm welcome to our new collaborator Patricia Hornitschek.
2020
- Our new QuantStudio 7 is now operational.
2019
- Our 10X Genomics Visium spatial transcriptomics service is launched. A fruitful collaboration with the HCF.
- Our 10X Genomics single cell ATAC-seq service is operational.
April 2024
- Visium HD is here! Spatial transcriptomics resolution is now down to single cell scale, more info here!
March 2024
- Our new Aviti sequencer is operational. Please inquire. More news soon anyway.
January 2024
- A warm welcome to our new collaborator Michael Stock.
November 2023
- New price list available here, with decrease of fixed RNA 10XG (FLEX) prices
October 2023
- Launch of our PromethION long reads service.
July 2023
- New price list available here! Many new services included. Price decrease of NovaSeq runs.
June 2023
- We have launched the 10X Genomics https://www.epfl.ch/research/facilities/gene-expression-core-facility/page-112636-en-html/page-153363-en-html/FLEX (fixed RNA profiling) service, based on fixed cells. Ideal for evening or weekend processing of samples since fixed cells can be stored.
April 2023
- We have just launched a HiC libraries prep service, based on Arima workflow. Inquire for pricing and details.
- A new libraries submission form is available here (together with updated guidelines).
February 2023
- Our new CytAssist instrument now allows us to process FFPE slides for spatial transcriptomics analysis of human and mouse tissues with improved sensitivity, flexibility and robustness.
- Our new QIAcuity One 5plex digital PCR system is up and running.
2022
- From now on please use our team email address [email protected] for projects submissions and other demands.
- New versions of our libraries submission form/guidelines are available here.
- We have launched our NovaSeq 6000 service! Please inquire.
2021
- We have launched a Visium FFPE spatial transcriptomics service, in collaboration with HCF.
- A NovaSeq 6000 will soon replace our HiSeq 4000! More news in December.
- A warm welcome to our new collaborator Chiara Sartori.
- The replacement process for our HiSeq 4000 sequencer has begun, more news in 2 months.
- Our new 10X Genomics single cell multiome (scATAC-seq+scRNA-seq) and CellPlex services are operational.
- Our new QuantStudio 6 instrument is now operational.
- Our new web tool CloneMap for ORFs browsing and QC is operational (thanks Fabrice David).
- A warm welcome to our new collaborator Patricia Hornitschek.
2020
- Our new QuantStudio 7 is now operational.
2019
- Our 10X Genomics Visium spatial transcriptomics service is launched. A fruitful collaboration with the HCF.
- Our 10X Genomics single cell ATAC-seq service is operational.
2018
- Our new HiSeq 4000 sequencer just arrived! Service will begin later in January.
- We released libraries submission guidelines for NextSeq sequencing. They are available here.
- We released RNA submission guidelines for RNA-seq, and gDNA submission guidelines for whole genome sequencing or exome sequencing. They are available here.
- A slightly updated version of our price list is available here.
- Our new 10X Genomics Chromium instrument is operational. This allows scaling up single cells transcriptomics experiments to thousands of cells.
- New user-friendly short protocols are available for the TapeStation 4200.
2017
- The GECF now provides bioinformatics services, presented in a dedicated web site. More news soon.
- New method available on our Fragment Analyzer for gDNA and large DNA fragments (up to 50 Kb).
- Release of user friendly quick start guides for our QuantStudio 6 and 7900HT qPCR instruments.
- New more user-friendly releases of our ORFs databases.
- A warm welcome to our new team members Elisa Cora and Lionel Ponsonnet!
2016
- Initial release of our new MOUSAIC collection of murine ORFs.
- New improved versions of the Fragment Analyzer kits were released.
- Our new Agilent TapeStation 4200 has arrived. This RNA/DNA profiling instrument nicely complements our Fragment Analyzer. Please contact us for more details.
2015
- Our next-generation Illumina NextSeq 500 sequencer is now operational. Please contact us for more details.
- New user-friendly short protocols are available for the Fragment Analyzer.
- Our service of automated 96-well plate minipreps is now available. Please inquire for further details.
- The new FlexSix system is available on our BioMark HD, allowing for much more flexible samples/genes combinations.
- CAHOS database v1.004 released, including 32 new human ZNF ORFs.
- Our new QuantStudio 6 Flex qPCR instrument is available for booking (only with a 384w block for now).