A 10X Genomics Chromium X allows the GECF to address diverse biological questions by generating transcriptomics and/or chromatin accessibility data for thousands of single cells. You can either bring us cells and we’ll perform the experiment, or you can rent the instrument and perform everything on your side (with your reagents). Our price list is available here.
Classical (non-FLEX) transcriptomics methods
A submission form is available HERE. Fill it as well as possible and send it to us before the experiment.
Samples submission guidelines for the classical 3′ scRNA-seq are available HERE.
An addendum regarding 5′ scRNA-seq +VDJ is available here.
For multiplexing:
- Totalseq antibodies-based method for 3′ and 5′ scRNA-seq multiplexing are available (ask us for details). A sandbox calculation file is available here.
- The new labelling-free “on-chip multiplexing” (OCM) solution for 3’GE and 5’GE is also available. An addendum on OCM is available here.
FLEX transcriptomics methods
FLEX allows to fix cells and store them before processing, specially useful for samples obtained late in the evening or during weekends. Quality is as good as classical 3’GE method, for cheaper, but is only available fo mouse and human samples.
A submission form for 10XG FLEX experiments is available HERE. Fill it as well as possible and send it to us before the experiment.
Samples submission guidelines are available HERE.
ATAC/multiome methods
Samples submission guidelines for multiome (scATAC+scRNA-seq) are available here. An introduction is available here.
Standalone scATAC-seq is also possible, guidelines are here.
The submission form is the same as for calssial (non-FLEX) transcriptomics methods.
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Please contact us for more information regarding other 10X Genomics methods, e.g. CITE-seq (co-quantification of cell surface proteins) or CRISPR screens.