A 10X Genomics Chromium X instrument allows the GECF to address diverse biological questions by generating transcriptomics and/or chromatin accessibility data for thousands of single cells. You can either bring us cells and we’ll perform the experiment for you, or you can rent the instrument and perform everything on your side (with your reagents). Our price list is available here.
Classical (non-FLEX) methods
A submission form for 10X Genomics experiments is available here. Fill it as well as possible and send it to us before the experiment.
Samples submission guidelines for the classical 3′ scRNA-seq are available here (or here for the older v3.1 version).
An addendum regarding 5′ scRNA-seq +VDJ is available here.
For multiplexing:
- Details regarding the 3′ scRNA-seq multiplexing solution CellPlex are available here. A sandbox calculation file is available here. Only available for 3’GE v3.1, therefore soon obsolete.
- Details regarding the totalseq antibodies-based method for 3′ and 5′ scRNA-seq multiplexing will be available here asap. A sandbox calculation file is available here. This multiplexing is compatible with both 3’GE v4 and 5’GE v2.
- 10XG will release a new multiplexing solution for 3’GE v4 and 5’GE v2 very soon. This will not necessitate any labelling/tagging. More news as soon as it is available end of November.
Samples submission guidelines for multiome (scATAC+scRNA-seq) are available here. An introduction is available here. Standalone scATAC-seq is also possible, guidelines are here.
Please contact us for more details regarding these guidelines, or for more information regarding other 10X Genomics methods, e.g. CITE-seq (co-quantification of cell surface proteins) or CRISPR screens.
FLEX methods
FLEX allows to fix cells and store them before processing, specially useful for samples obtained late in the evening or during weekends. Quality is as good as classical 3’GE method, for cheaper, but is only available fo mouse and human samples, and is not yet compatible with ATAC/multiome/VDJ. A submission form for 10XG FLEX experiments is available here. Fill it as well as possible and send it to us before the experiment.
Samples submission guidelines for FLEX are available here.
New: FLEX is now also compatible with concomitant protein quantification through oligo-tagged antibodies. Inquire for more details.
For Spatial Transcriptomics, please refer to our dedicated page.