The GECF offers a 10X Genomics Visium and Visium HD Spatial Transcriptomics service with the Histology Core Facility. This allows for transcriptome-wide mapping of genes expression onto tissue sections, down to single cell-like level and with concomitant H&E or IF staining.
When mouse of human tissues are studied, the CytAssist instrument enables processing fresh frozen, fixed frozen or FFPE slides with improved sensitivity and robustness. Other advantages include an option for bigger tissues section (11mm x 11mm), the ability to select an area of interest within a larger tissue section, and the ability to process tissues microarrays.
An overview of the different available Visium methods is available HERE.
Our guidelines and submission forms are available here:
- non-HD polyA (all eukaryotes): GUIDELINES, FORM.
- non-HD Cytassist (mouse+human): GUIDELINES, FORM.
- HD Cytassist (mouse+human): GUIDELINES, FORM.
In the (not recommended) case where you would not go through the EPFL Histology Core for slides preparation, we have additional histo guidelines for the non-HD polyA-based workflow HERE, for the non-HD Cytassist HERE, and for Cytassist HD HERE. Please contact us for more details.
Localizing a pre-designed panel of proteins in parallel is also possible, owing to oligo-tagged antibodies (for now only available for a human immune markers panel). More info HERE.
In case Visium is not suited for your needs, we also offer the Curio methods, such as slide-seq (Curio seeker) or slide-tags (Curio Trekker).
Finally, the SpatialOmics@EPFL web page presents a comprehensive overview of the other Spatial Omics options available at the School of Life Science.