|
Staining |
Tissues |
Results |
|
|
AB |
Alcian blue |
Intestine |
Acid mucins – blue |
|
AB/ PAS |
Alcian Blue & Periodic acid Schiff |
Acid mucins – blue Neutral mucins – magenta |
|
|
AB/ VG |
Alcian Blue & Van Gieson |
Acid mucins – blue Collagen – pink Muscle and others – yellow |
|
|
CR |
Congo red |
Amyloid, eosinophil & elastin – red |
|
|
CV |
Cresyl violet |
Nervous system |
Nuclei and nissl substance – purple |
|
CV/ LB |
Cresyl Violet/Luxol fast blue |
Nervous system |
Myelin – blue Red blood cells – turquoise Nuclei and nissl substance – purple |
|
FM |
Fontana Masson |
Skin, brain |
Melanin (also some lipofuscins) – black |
|
G |
Giemsa |
Blood cells and parasites |
Acidophils – pink/red Basophils – blue Eosinophils – red/orange Mast cells – purple Parasites – blue/dark blue |
|
GC |
Golgi Cox |
Brain |
Cortical neurons and processes |
|
GMS |
Grocott methenamine silver |
Any tissue containing fungus |
Fungi – black Background – green |
|
GT |
Goldner trichrome |
Skin Nervous system |
Cytoplasm, keratin, muscle fibres, intercellular fibres – red Collagen, mucins – green |
|
JMS |
Jone’s methenamine silver |
Kidney |
Basement membrane of glomeruli – black Background – green |
|
MT |
Masson trichrome |
All tissues |
Cytoplasm, keratin, muscle fibres, intercellular fibres – red Collagen, mucins – blue |
|
ORO |
Oil red O |
Cryosections of adipocytes |
Lipids – bright red |
|
PAS |
Periodic acid Schiff |
Intestine (goblet, paneth cells), mucous gland |
Periodate carbohydrates – magenta |
|
PB |
Prussian blue |
Ferric ion – blue |
|
|
RF |
Resorcin Fuchsine |
Ealstic fibers – violet Muscle and others – yellow |
|
|
TB |
Toluidine blue |
Mast cell granules – red/purple |
|
|
VG |
Van Gieson |
Collagen – pink Muscle and others – yellow |
|
|
VGRF |
Van Gieson & Resorcin Fuchsin |
Elastic fibers – blue/black Collagen – pink/red Muscle and others – yellow |
