LabView CSU Microdissection

Labview CSU Microdissection Microscope
Pulsed UV laser microdissection with confocal spinning disk unit.

Custom-built microscope capable of <3um diameter microablation using a pulsed UV laser. Attached to confocal spinning disk unit for fast optical slicing.

Person in charge: Olivier Burri, José Artacho

Book on SV-BBS

Technical Specifications

Stand: Inverted Zeiss Axiovert 200 Motorized
XY-Stage: Manual
Illumination: Transmitted light, laser
Software: LabView, custom BIOP software

Detectors

CamerasRoper CoolSNAP HQ
Chip TechnologyCCD Grayscale
Sensor Format 1392 x 1040
Chip size8.71 mm x 6.90 mm
Pixel size6.45 um x 6.45 um
Dynamic Range12-bit (36-bit color)
C-mount1.0x

Objectives

ObjectiveMag/NAMediumContrastWD (cm)
AchroPlan10x/0.25air–4.80
Plan Neofluar20x/0.50air–2.00
N-Achromat63x/.90 corrwaterDIC 2.40

Laser Lines

WavelengthLaser Type
488 nmArgon

Filtercubes

NameExcitationDichroicEmissionNo
GreenBP 450-490FT 510LP 515FS 09