Leica SP8 STED Microscope, with hybrid photon counting detectors (HyD). Capable of Stimulated Emission Depletion (STED) for 3D superresolution up to 40nm.
Boasts full tempterature and CO2 Control and a pulsed supercontinuum tunable white light laser.
Person in charge: José Artacho & Thierry Laroche
Book on SV-PPMS
Technical Specifications
Stand: Inverted DMI6000 motorized
XY-Stage: Motorized XYZ
Illumination: Lumencor Sola LED and Halogen Lamp
Software: LEICA LAS-X
STED Module
Depletion Lasers:
- 592 nm
- 660 nm
- 775 nm (pulsed)
Detectors
| Detector Type | Amount |
| PMT | x3 (range: 390nm to 790nm) |
| HyD | x2 (range: 390nm to 790nm) |
| Transmission PMT | x1 |
Objectives
| Objective | Mag/NA | Medium | Contrast | WD (mm) |
| HC PL FLUOTAR | 10x/0.30 | air | 11.00 | |
| HC PL Fluotar | 25x/0.95 | water* | 2.40 | |
| HC PL APO | 63x/1.40 | oil | DIC | 0.14 |
| HC PL APO Corr | 93x/1.30+ | glyc** | DIC | 0.30 |
| HC PL APO Corr | 100x/1.40+ | oil | DIC | 0.13 |
+ Used for STED imaging.
** Motorized correction ring. * With water dispenser
Laserlines
| Wavelenghts | Laser Type |
| 405 nm | Diode |
| Supercontinuum laser | Range: 470 nm – 670 nm |
Filtercubes
The links in the table below will send you to an FPbase configuration page.