Zeiss Lightsheet Z1 dual sided selective plane illumination microscope (SPIM). Can image live samples for long periods with full temperature and CO2 control. Includes chambers for imaging cleared samples.
Person in charge: Thierry Laroche, Olivier Burri
Book on SV-PPMS
Technical Specifications
Stand: Lateral mounted, Top-fed Zeiss Lightsheet Z1
XY-Stage: Motorized for XYZ and Rotation
Illumination: Laser
Software: ZEN Black
Detectors
| Cameras | 2x PCO Edge 5.5 |
| Chip Technology | CMOS Grayscale |
| Sensor Format | 2560 x 2160 |
| Chip size | 16.6 mm x 14.4 mm |
| Pixel size | 6.5 um x 6.5 um |
| Dynamic Range | 16-bit |
| C-mount | Internal Zoom Optics |
Objectives
| Objective | Mag/NA | Medium | Contrast | WD (mm) |
| EC Plan Neofluar | 5x/0.16 | air | – | 18.50 |
| W Plan Apochromat | 20x/1.0 corr | water | – | 1.80 |
| W Plan Apochromat | 40x/1.0 | water | – | 2.50 |
| W Plan Apochromat | 63x/1.0 | water | – | 1.80 |
| EC Plan Neofluar | 20x/1.45 corr | clearing | – | 5.60 |
Lasers
| Wavelenghts | Laser Type |
| 405 nm | Solid state |
| 488 nm | Solid state |
| 555 nm | Solid state |
| 639 nm | Solid state |
Filtercube Pairs
The filtercubes on the system work in wavelength pairs in order to perform dual camera acquisitions.
| Position | Camera 1 Wavelength | Camera 2 Wavelength | Dye Pairs |
| 1 | Mirror | Nothing | Brightfield |
| 2 | BP 420-470 | BP 525-545 | DAPI-YFP |
| 3 | BP 420-470 | BP 575-615 | DAPI-RFP |
| 4 | BP 505-545 | BP 575-615 | GFP-RFP |
| 5 | BP 505-545 | LP 585 | GFP-mCherry |
| 6 | BP 505-545 | LP 660 | GFP-Draq5 |