Zeiss LSM 980 Inverted microscope. Multi-purpose confocal with 32 Channels AiryScan, tPMT, widefield & brightfield capability. Motorized stage. LIS Brick and Cube for temperature and humidity control.
Persons in charge: Nicolas Chiaruttini
Book on SV-PPMS
Technical Specifications
Imaging: Confocal & Widefield
Stand: Inverted Axio Observer
XY-Stage: Motorized XYZ
Illumination: 100W Hal (transmission), Colibri 5 for (fluorescence)
Software: ZEN Blue ver. 3.4.91
Detectors
2 photomultiplier tubes (PMT) for fluorescence 4 GaAsP detectors for fluorescence
2 high sensitivity near infrared (NIR) detector for fluorescence
1 Airyscan (32 channel PMT) for fluorescence
1 transmission photomultiplier tube (tPMT) for brightfield
| Camera | Axiocam 506 mono |
| Chip Technology | CCD Grayscale |
| Sensor Format | 2752 x 2208 |
| Chip size | 12.5 mm x 10.0 mm |
| Pixel size | 4.54 um x 4.54 um |
| Dynamic Range | 14-bit |
| C-mount | 1.0x |
Objectives
| Objective | Mag/NA | Medium | Contrast | WD (mm) |
| EC Plan-Neofluar | 10x/0.30 | air | – | 5.2 |
| Plan-Apochromat | 20x/0.80 | air | DIC | 0.55 |
| LD LCI Plan Apochromat | 25x/0.80 Corr | Imm | DIC | 0.57 at 0.17 |
| LD LCI Plan Apochromat | 40x/1.20 autocorr | Imm | DIC | 0.41 at 0.17 |
| Plan-Apochromat | 63x/1.40 | oil | DIC | 0.19 |
Laser Lines
| Wavelength | Laser Type |
| 405 nm | Diode |
| 445 nm | Diode |
| 488 nm | Diode |
| 561nm | DPSS |
| 639 nm | Diode |
| 730 nm | Diode |
Filtercubes
The links in the table below will send you to an FPbase configuration page.
Tutorials
Confocal Tutorial
Incorrect dimensions
Airy Scan Tutorial
Incorrect dimensions
40x IMM Korr Tutorial
Incorrect dimensions