Pulsed UV laser microdissection with confocal spinning disk unit.
Custom-built microscope capable of <3um diameter microablation using a pulsed UV laser. Attached to confocal spinning disk unit for fast optical slicing.
Person in charge : Olivier Burri , José Artacho
Technical Specifications
Stand: Inverted Zeiss Axiovert 200 MotorizedXY-Stage: ManualIllumination: Transmitted light, laserSoftware: LabView, custom BIOP software
Detectors
Cameras Roper CoolSNAP HQ Chip Technology CCD Grayscale Sensor Format 1392 x 1040 Chip size 8.71 mm x 6.90 mm Pixel size 6.45 um x 6.45 um Dynamic Range 12-bit (36-bit color) C-mount 1.0x
Objectives
Objective Mag/NA Medium Contrast WD (cm) AchroPlan 10x/0.25 air – 4.80 Plan Neofluar 20x/0.50 air – 2.00 N-Achromat 63x/.90 corr water DIC 2.40
Laser Lines
Wavelength Laser Type 488 nm Argon
Filtercubes
Name Excitation Dichroic Emission N o Green BP 450-490 FT 510 LP 515 FS 09